Microbiological Analysis of Crevicular Fluid to Determine Presence of Candida albicans in Periodontal Pockets of Adult Subjects

2022 • Volume 16 • Issue 1

Fabiola Gutiérrez Romero; Milagritos Moreno Sosa; César-Augusto Padilla-Avalos & Rafael Morales-Vadillo

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Summary

The aim of this work was to establish the association between the depth of the periodontal pocket and the presence of Candida albicans in the crevicular fluid of adult subjects with gingival health and periodontitis, received in the area of the Master in Periodontology of a Dental Center. The study consisted of 81 samples of crevicular fluid obtained with a No. 40 paper cone. They were distributed into three groups: G1: 20 samples from the control group (gingival health), G2: 32 samples from pockets with depth of 4-6 mm and G3: 29 samples from pockets with depth >6 mm; which were placed in Sabouraud Dextrose Broth for transport to the laboratory, where it was cultivated in Sabouraud Dextrose Agar for 48h at 37 ºC. Subsequently, colony staining and a germ tube test were performed to confirm the presence of Candida albicans. Of the total samples, 12 (14.8 %) showed the presence of Candida albicans, of which 6 (18.8 %) belonged to the group of pockets with depth of 4-6 mm and the other 6 (20.7 %) belonged to the group of bags with depth> 6mm. In the control group, no presence of Candida albicans was observed. The patients with gingival health did not present Candida albicans. However, Candida albicans was present in patients with periodontal pockets of 4-6 mm and >6 mm (14.8 %). No statistically significant difference was found between the presence of Candida albicans with depth of the periodontal pocket (p=0.849).

KEY WORDS: Candida albicans, periodontitis, periodontal pocket.

 

How to cite this article

GUTIÉRREZ, R. F.; MORENO, S. F.; PADILLA-AVALOS, C. A. & MORALES-VADILLO, R. Microbiological analysis of crevicular fluid to determine presence of Candida albicans in periodontal pockets of adult subjects. Int. J. Odontostomat., 16(1):1-6, 2022.

 

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