Oncocytic Proliferations of Salivary Glands: Structural and Immunohistochemical Study of 7 Cases

2019 • Volume 13 • Issue 1

Rodolfo Esteban Avila; María Elena Samar; Ismael Bernardo Fonseca; Alberto Gustavo Corball; Víctor Carriel; Laura García-Martinez & Ismael Rodríguez



Oncocytes are cells probably originated by metaplastic transformation of the ductal or acinar epithelium of parotid and submandibular. Its proliferation can cause pathological conditions that include multinodular adenomatous oncocytic hiperplasia (HOAM), oncocytomas and oncocytic carcinomas. Oncocytic tumors make up 1 % of all salivary tumors and between 82 and 90 % develop in the parotid; the rest of the tumors are divided between the submandibular gland and the minor salivary glands. Multinodular oncocytic hyperplasias are extremely rare. In the present work we analyzed five cases of parotid oncocytomas and two cases of HOAM, one of parotid and the other of submandibular; structural and immunohistochemical characteristics of the oncocytes were described. Biopsies were included in paraffin, serial cuts were stained with H&E, Hematoxylin / phosphotungstic acid (PTA / H), PAS and were marked with antimitochondrial AC, CK 5/6, CKs 20 and EMA. The tumors showed a nodular growth encapsulated by dense connective tissue. The histological cuts showed dark eosinophilic oncocytes and violaceous granulations with PTA / H. In two cases of parotid and the case of submandibular HOAM, PAS positive clear oncocytes were also present. The immunostaining was positive in all the cells, being the labeling for peripheral mitochondria in the clear oncocytes. Eosinophilic cells PTA / H positive with strongly marked with antimitochondrial AC, CKs and EMA confirm the diagnosis of oncocytic pathology. In three cases, light and dark oncocytes coexist. Clear cells are oncocytes that accumulate glycogen in their cytoplasm, displacing the mitochondria to the periphery. In the differential diagnosis we should consider salivary tumors with clear cells, metastatic renal carcinoma, Whartin’s tumor, the clear cell variant of epithelial / myoepithelial carcinoma and mucoepidermoid carcinoma with oncocytic metaplasia.

KEY WORDS: salivary glands, oncocytes, oncocitoma, multinodular adenomatous oncocytic hyperplasia, histology, immunohistochemistry.


How to cite this article

AVILA, R. E.; SAMAR, M. E.; FONSECA, I. B.; CORBALL, A. G.; CARRIEL, V.; GARCÍA-MARTINEZ, L. & RODRÍGUEZ, I. Oncocytic proliferations of salivary glands: structural and immunohistochemical study of 7 cases. Int. J. Odontostomat. 13(1):82-88, 2019.


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