Identification of the Salivary Microbiota of Patients with Oral Cancer in Antofagasta – Chile by Molecular Diagnosis of the 16S rRNA Gene

2018 • Volume 12 • Issue 1

Lizama, Catherine; Valenzuela, Oriana; Mejia, Magaly



Oral cancer, is part of several cancers that affect head and neck, and that according to numerous studies shows an increase of approximately 3-5 % of all cancers. In the last 10 years there has been an increased interest in saliva for its use as a diagnostic tool, due to its easy access, and non-invasive sample collection, unlike blood samples. Studies of metagenomics to the microbiota of oral cancer patients have shown the presence of some groups in greater proportion, it is postulated that they could be used as markers especially in the saliva of these patients. In Antofagasta the number of patients with this type of cancer increases each year. However, there are no studies on the changes that affect the oral microbiota in these patients. The objective of this study is to contribute to the knowledge of the microbiota present in the saliva of patients with oral cancer in the region of Antofagasta, limiting this study exclusively to the group of cultivable anaerobic bacteria. The strains identified are related to the group of Streptococcus mitis / oralis, S. anginosus and S. gordoni, only one strain is grouped near the cluster that includes S. australis and S. parasanguinis. 36.4 % of the molecularly identified isolates, are closely related to the S. anginosus species described in the literature in oropharyngeal tumors. The same percentage is obtained for the taxa related to the cluster that includes S. mitis, proposed in other studies as a diagnostic indicator in subjects with oral squamous cell carcinoma.

KEY WORDS: oral microbiota, saliva, oral cancer, microbiological indicators.


How to cite this article

LIZAMA, C.; VALENZUELA, O. & MEJIA, M. Identification of the salivary microbiota of patients with oral cancer in AntofagastaChile by molecular diagnosis of the 16s rRNA gene. Int. J. Odontostomat., 12(1):87-92, 2018.


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