Effect of Two Probiotics Containing Lactobacillus casei rhamnosus and Lactobacillus johnsonii variety on the in vitro Growth of Streptococcus mutans

2013 • Volume 7 • Issue 3

M. Rebolledo; E. Rojas & F. Salgado

DOI:

Summary

Dental caries is one of the most prevalent diseases in the world and even more in the population of developing countries, so it is necessary to use new measures for prevention. Previous studies indicate that the effect of probiotic strains inhibit colonization of pathogens responsible for dental caries such as Streptococcus mutans, preventing the onset of dental caries. The aim was to measure the effect of the probiotic strains Lactobacillus casei variety rhamnosus (LCR32) and Lactobacillus johnsonii (LA1) on in vitro growth will Streptococcus mutans. We measured the effect in vitro of two probiotic strains marketed in Chile; Lactobacillus casei variety rhamnosus (LCR32) contained in Lactyl ® and Lactobacillus johnsonii (LA1) contained in Chamyto on in vitro growth of Streptococcus mutans. Culture media were made selective for Streptococcus mutans to which were added four different dilutions of each probiotic and measured the zone of inhibition of Streptococcus mutans with a foot tube. The probiotic strains Lactobacillus casei variety rhamnosus (LCR32) and Lactobacillus johnsonii (LA1) inhibit the growth of Streptococcus mutans. Both probiotics in the two higher concentrations no significant differences in relation to the zones of inhibition. However, the probiotic strain Lactobacillus casei rhamnosus variety (LCR35) showed the most significant zones of inhibition compared to the strain Lactobacillus johnsonii (LA1). The probiotic strains Lactobacillus casei rhamnosus variety (LCR35) and Lactobacillus Johnosonii (LA1) decreased the colonization of the main dental caries producing bacteria, so these probiotic could be used as support in the prevention and prophylaxis of the disease in high-risk cariogenic patients, additionally in other means of prevention.

KEYWORDS: caries, probiotic, bacterial strains, Lactobacillus, Streptococcus mutans.

 

How to cite this article

REBOLLEDO, M.; ROJAS, E. & SALGADO, F. Effect of two probiotics containing Lactobacillus rhamnosus and Lactobacillus johnsonii variety on the in vitro growth of Streptococcus mutans. Int. J. Odontostomat., 7(3):415-419, 2013.

 

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