IL-1B (+3954C>T) Gene Polymorphism and its Association with External Root Resorption after Orthodontic Treatment

Fanny Lince Vides; Julio De La Ossa Salcedo; Rubi Hernandez Tirado; Yaleyvis Buelvas Montes; José Bustillo Arrieta & Meisser Madera Anaya

Summary

The objective of this study was to determine the presence of Interleukin 1 beta (IL-1B) rs1143634 (+3954C>T) gene polymorphism and its association with external apical root resorption (ERR) after orthodontic treatment. We conducted a pilot study of individuals treated with orthodontic treatment, 13 (cases) had ERR after orthodontic treatment and 22 (controls) were clinically healthy. DNA was extracted from samples of epithelial cells from the oral cavity and IL-1B rs1143634 (+3954C>T) gene polymorphism was genotyped by polymerase chain reaction and digestion product through the TaqI restriction enzyme. Genotype and allele frequencies of rs1143634 were estimated; in addition, the deviation from Hardy-Weinberg equilibrium was assessed. Allele and genotype frequencies were compared using the c2 test with likelihood ratio (p <0.05). The mean age of participants was 28.6 (SD= 11.5) years and 68.6 % were females. No statistically significant association was found between the genotypes distribution of IL-1B (+3954C>T) polymorphism with ERR (p= 0.0926). However, a significant difference in the alleles distribution (p= 0.035) was observed, where the allele T (allele 2) was more prevalent in the control group. IL-1B (+3954C>T) polymorphism was present in the study population. Although there were no differences in the genotypes distribution to support an association between this polymorphism with ERR, there was a difference in the alleles distribution, suggesting that the allele T possibly acts as a protective factor against the development of ERR.

KEY WORDS: external root resorption, apical resorption, Interleukin-1 beta.

How to cite this article

LINCE, V. F.; DE LA OSSA, S. J.; HERNANDEZ, T. R.; BUELVAS, M. Y.; BUSTILLO, A. J. & MADERA, A. M. IL-1B (+3954C>T) gene polymorphism and its association with external root resorption after orthodontic treatment. Int. J. Odontostomat., 10(2):243-248, 2016.