Early Detection of Periodontal Pathogens by PCR in Relation to Osseointegrated Implants

Iván Godoy; Juan Pablo Parrochia; Mauricio Bittner & José Valdivia

Summary

The aim of this study was to assess the early colonization of four periodontopathogenic bacteria (A. actinomycetemcomitans, P. gingivalis, T. forsythensis and T. denticola) on titanium implants immediately after two weeks following second stage surgery; to compare the presence of these four periodontopathogenic bacteria at subgingival implant and adjacent tooth sites. Subgingival plaque samples from implant and neighboring teeth were studied by PCR after two weeks following second stage surgery. Sixteen implants and thirteen teeth, in ten patients were selected. At two weeks, pathogenic bacteria presence was found in both peri-implant and periodontal sites, there was no relation found between tooth and implant for P. gingivalis, T. forsythensis and T. denticola. A significant relation was found for A. actinomycetemcomitans (P<0.005). The more frequently detected bacteria were P. gingivalis, and the less was T. denticola. The dependence for A. actinomycetemcomitans was related to patients with a history of periodontitis. Within the limits of this study, the findings showed the early presence of the four periodontopathogenic bacteria around implant sites and a statistically significant (P<0.005) relation between implants and teeth sites for A. actinomycetemcomitans.

KEY WORDS: periodontal pathogens, PCR, osseointegrated Implants.

How to cite this article

GODOY, I.; PARROCHIA, J. P.; BITTNER, M. & VALDIVIA, J. Early detection of periodontal pathogens by PCR in relation to osseointegrated implants. Int. J. Odontostomat., 8(3):405-412, 2014.