Efraín Álvarez Martínez; Raul Jiménez-Gómez & Carlos Martin Ardila Medina
Summary
Molecular biological markers have been suggested to be of value in the diagnosis and prognostic evaluation of precancerous lesions. The purpose of the current study was to investigate the expression of p53 protein in normal oral mucosa, oral dysplastic lesions (DL), and oral squamous cell carcinoma (SCC), comparing patients with the habit of reverse smoking and conventional smokers. The patients were subjected to incisional biopsy and the soft tissue specimens were routinely fixed in 10% formalin and processed in the laboratory. Immunohistochemical technique was performed using the avidin-biotin-peroxidase protocol. The 102 patients included 42 reverse smokers and 60 conventional smokers. There were 79 cases of mild, 15 of moderate, and 3 of severe epithelial dysplasia. Five microinvasive oral SCC included one male and four females. The clinical characteristics of microinvasive tumors included patches, plaques or erosions; 100% showed positive nuclear staining for p53. It was found a significant association of p53 expression and exposure of reverse smoking among microinvasive oral SCC and oral DL patients in the population studied.
KEY WORDS: p53, immunohistochemistry, oral squamous cell carcinoma, reverse smoker.
How to cite this article
ÁLVAREZ, M. E.; JIMÉNEZ-GÓMEZ, R. & ARDILA, M. C. M. Immunoexpression of p53 in oral squamous cell carcinoma and oral dysplastic lesions in patients with the habit of reverse smoke. Int. J. Odontostomat., 7(2):185-191, 2013.