Adhesion and Initial Colonization of Streptococcus mutans is Influenced by Time and Composition of Different Composites

Raquel A. B. Silva; Paulo Nelson-Filho; Katharina M. H. De Oliveira; Priscilla C. Romualdo; Patrícia Gatón-Hernandez; Carolina P. Aires & Lea A. B. Silva

Summary

The aim of this study was to evaluate the adhesion and initial colonization of S. mutans on the surface of novel composite resins during three experimental periods. Biofilms were formed on specimens of Enamel Plus HRI (Hri), FiltekTM Silorane (Si), Tetric EvoCeram® Bulk Fill (Te), KaloreTM (K), and FiltekTM Z250 (Z) (n=4/experimental period). After 4, 8 and 24 hours, the specimens were assessed for bacterial colony forming unit (CFU/mm2) levels and scanned by electron microscopy. All material surfaces showed a similar susceptibility to bacterial adhesion at 4 hours (p>0.05). The amount of microorganisms in the formed biofilms increased at 8 hours for all groups (p<0.05) and decreased at 24 hours only for Te and Z groups (p<0.05). After 24 h, the K group showed higher microorganism counts compared to the other groups. All composite resins evaluated were susceptible to adhesion by streptococci. However, bacteria attachment was not sufficient to maintain S. mutans colonization on Tetric EvoCeram® Bulk Fill and FiltekTM Z250 composites. Composite K presented the highest mean values for bacterial adhesion. Also, it was observed that the composition of resins could interfere with colonization mechanisms.

KEY WORDS: composite resins; biofilm, streptococcus mutans, electron microscopy.

How to cite this article

SILVA, R. A. B.; NELSON-FILHO, P.; DE OLIVEIRA, K. M. H.; ROMUALDO, P. C.; GATÓN-HERNANDEZ, P.; AIRES, C. P. & SILVA, L. A. B. Adhesion and initial colonization of streptococcus mutans is influenced by time and composition of different composites. Int. J. Odontostomat., 12(4):395-400, 2018.