In vitro Study of the Antibacterial Effect of Oleoresin of Copaifera reticulata and the Essential Oil of Origanum majoricum against Streptococcus mutans and Enterococcus faecalis Bacteria of Importance in Oral Pathologies

2018 • Volume 12 • Issue 4

Hilda Moromi Nakata; Donald Ramos Perfecto; Jorge Villavicencio Gastelumendi; Elba Martínez Cadillo; Alejandro Mendoza Rojas; Edith Chavez Alvarado; Lita Ortiz Fernández & Ángela Quispe Salcedo



The objective of the study was to determine the in vitro antibacterial effect of the oleoresin of Copaifera reticulata (C. reticulata) “copaiba” and of the essential oil of Oreganum majoricum (O. majoricum) “oregano” against Streptococcus mutans (S. mutans) and Enterococcus faecalis (E. faecalis). Sensitivity tests were developed by first activating the bacteria strains to be confronted. The oleoresin of copaiba was diluted with dimethylsulphoside (DMSO), obtaining final concentrations to be tested of 100 %, 50 %, 25 %, and 12.5 %. In relation to the essential oil of oregano, it was only 100 % tested. For the disk agar diffusion test, 100 mL of each bacterial strain was taken at a turbidity of 0.5 of Mc Farlam, to be planted by dissecting trypticase soy agar plates, and then placing the disks equidistantly loaded with the different concentrations of natural products; 0.12 % chlorhexidine was used as a positive control and DMSO as negative control. The plates were incubated by the candle method in extinction at 37 °C, for a period of 24 hours, after which time the inhibition halos were read. The results obtained by the copaiba, determined an antibacterial effect in its four concentrations, being the biggest halos of inhibition at the concentration of 100 %, copaiba genus higher average halos for S. mutans of 30.00 ± 0.00 mm and for E. faecalis of 8.3 ± 0.50 mm. In the case of oregano, haloes are produced at a concentration of 100 % with an average of 25.3 ± 0.96 mm for S. mutans and for E. faecalis 9.5 ± 1.29 mm. It is concluded from the study that both copaiba and oregano present an antibacterial effect for both bacteria, being its greater antibacterial effect for both natural products on S. mutans.

KEY WORDS: Antibacterial agent, Enterococcus faecalis, Copaiva, Oreganum, Streptococcus mutans.


How to cite this article

MOROMI , N. H.; RAMOS, P. D.; VILLAVICENCIO, G. D.; MARTÍNEZ, C. E.; MENDOZA, R. A.; CHAVEZ, A. E.; ORTIZ, F L. & QUISPE, S. Á. In vitro study of the antibacterial effect of oleoresin of Copaifera reticulata and the essential oil of Origanum majoricum against Streptococcus mutans and Enterococcus faecalis bacteria of importance in oral pathologies. Int. J. Odontostomat., 12(4):355-361, 2018.


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