The aim was to evaluate the dissolution of porcine muscle using sodium hypochlorite in several concentrations and in combination with 3% hydrogen peroxide. Small pieces of porcine muscle were cut and their weight adjusted at 0.02g to be submerged in 1 ml of each test solution which was changed in 1 minute intervals until complete dissolution of the sample was observed. The 75 pieces of tissue were divided in 5 groups and different irrigant solutions were used as follows: Group 1, 5% sodium hypochlorite (NaOCl 5%). Group 2, 2.5% sodium hypochlorite (NaOCl 2.5%). Group 3, 2.5% sodium hypochlorite and 3% hydrogen peroxide alternately (NaOCl 2.5% + H2O2 3%). Group 4,3% hydrogen peroxide (H2O2), and group 5 used saline (NaCl 0.9%) as a negative control. For 5% sodium hypochlorite the mean time until complete dissolution of the sample was 17.5 minutes. The mean time for group 2 was 18.5, for group 3 was 64.6 minutes and for groups 4 and 5 there was no tissue dissolution after 120 minutes. To evaluate statistical significance ANOVA multi sample test was performed and Tukey post hoc test with significance level at P=0.05. Under the conditions of this study there were no significant differences with the use of sodium hypochlorite at 5% and 2.5% in terms of dissolution speed (P=0.6). The combination of sodium hypochlorite and hydrogen peroxide alternately caused a statistical significant delay in the dissolution of porcine muscle (P<0.05). The combination of sodium hypochlorite and hydrogen peroxide does not offer any benefit in terms of speed of dissolution of connective necrotic tissue.
KEY WORDS: sodium hipochlorite, irrigating solution, hydrogen peroxide.
How to cite this article
ECHEVERRI, D. & ACUÑA, C. Dissolution of connective tissue in sodium hypochlorite alone and in combination with 3% hydrogen peroxide. Int. J. Odontostomat., 6(3):263-266, 2012.