The objective of the study was to evaluate the antimicrobial effect of three endodontic sealant cements for Streptococcus mutans, Enterococcus faecalis and Candida albicans. The agar diffusion method was used, making wells 5mm in diameter by 4mm deep, where the different cements that were prepared according to the manufacturer’s instructions were placed. For S. mutans and E. faecalis, brain-heart agar was used, while for C. albicans trypticase soy agar was used as culture media. The plates were incubated for 48 hours at 37 ºC. The microbial growth inhibition halos were measured with a Vernier compass. The results showed significant differences between all groups p<0.05. The cement based on zinc oxide and eugenol (Endofill) showed the best antimicrobial effect against the three strains with inhibition halos of 23.283 ± 5.497 mm for S. mutans, 8.468 ± 0.755 mm for E. faecalis and 21.965 ± 1.187 mm for C. albicans. While the resin-based cement (Vioseal) had a similar activity to Endofill only for S. mutans (21.262 ± 2.602 mm) (p>0.05), being considerably lower for E. faecalis (6.642 ± 0.659 mm) and C. albicans (8.781 ± 0.735 mm). Calcium hydroxide-based cement (Sealer 26) was more effective against S. mutans (13.010 ± 2.006 mm) than against E. faecalis (6.165 ± 0.978 mm) and C. albicans (5.640 ± 0.280 mm). In conclusion, cement based on zinc oxide and eugenol (Endofill) showed the best antimicrobial effect against the three strains, followed by resin-based cements (Vioseal) and finally calcium hydroxide (Sealer 26).
KEY WORDS: Streptococccus mutans, Enterococcus faecalis, Candida albicans,dental cements.
How to cite this article
CHAMBILLA, T. K. & SÁNCHEZ-TITO, M. A. Antimicrobial effect of three sealant endodontic cements for Streptococcus mutans, Enterococcus faecalis and Candida albicans. Int. J. Odontostomat., 15(3):610-615, 2021.